Video: Organoids derived from high-risk primary and micro metastasis prostate as well as distant metastasis colorectal tumors. These cultures, derived from resected patient tissues, were established, treated with chemotherapy drugs ex vivo and imaged by Alexandre Matov at the University of California, San Francisco. Each organoid is a hundred to several hundred micrometers in diameter and is assembled by dozens of thousands of individual patient cells from slow-proliferating, genetically heterogeneous, drug-naïve tumors or aggressive, recurrent disease cells emerging after hormonal and radiation therapies. Small molecules and genetic manipulations, which are not feasible in vivo, can be performed in organoids, making them a unique, physiologically relevant resource for functional interrogation of drug response. The organoid culture system presents us with a fantastic opportunity to develop living biobanks for screening of panels of drugs approved for clinical use as well as discovery of novel compounds, which will provide the foundations of precision medicine and personalized patient care for all.
The focus of the Biomedical Computer Vision Laboratory (BCVL) is the computational analysis of microscopy images of cells and cellular processes. The head of the lab is Alexandre Matov, PhD.
BCVL is currently pursuing scientific projects in the areas of basic and translational research. These include several interdisciplinary collaborations encompassing computational science, applications of light microscopy, as well as cell and cancer biology. Our expertise is in the design and development of computer vision algorithms for quantification of fluorescence microscopy images, and in applying engineering principles and mathematical modeling to address human health and disease problems.
Presentation at the University of Information Science & Technology St Paul the Apostle, Ohrid: Motion analysis of fluorescently labeled polymerizing microtubule ends in interphase cancer cells treated with taxanes
Presentation at the Medical Faculty of the University Sts Cyril & Methodius, Skopje: Компјутерска анализа на динамиката на микротубулите за персонализирана хемотерапија
Presentation at the Center for Research in Computer Vision at the University of Central Florida, Orlando: Tracking antiparallel motion in microtubule & f-actin networks imaged by fluorescent speckle microscopy. Part II: Enumeration & analysis of circulating tumor cells from peripheral blood of metastatic prostate cancer patients.
Galletti G, Matov A, Beltran H, Fontugne J, Mosquera JM, Cheung C, MacDonald TY, Sung MS, O’Toole S, Kench JG, Chae SS, Kimovski D, Tagawa ST, Nanus DM, Rubin MA, Horvath L, Giannakakou P, Rickman DS. ERG induces taxane resistance in castration-resistant prostate cancer. Nature Communications, 2014 Nov 25;5:5548
Milunovic-Jevtic A, Hazel JH, Groen AC, Ishihara K, Marina N, Mitchison TJ, Matov A, Gatlin JC. Investigating microtubule growth dynamics in discrete cytoplasmic volumes. Molecular Biology of the Cell 24, American Society for Cell Biology 2013
Matov A, Kimovski D, Galletti G, Pera B, Harkcom WT, Rickman DS, Giannakakou P. Computational analysis of microtubule dynamics for personalized cancer therapy. Cancer Research, 73 (8), American Association for Cancer Research 2013
Sung MS, Giyrezi A, Lee GY, Matov A, Galletti G, Loftus M, Syed Y, Lannin T, Hristov A, Mason C, Tagawa ST, Kirby B, Nanus DM, Giannakakou P. Using circulating tumor cells (CTCs) to interrogate mechanisms of taxane resistance in the prospective TAXYNERGY clinical trial in prostate cancer. Cancer Research, 73 (8), American Association for Cancer Research 2013
Matov A, Marina N. Analysis of unstructured crowded scenes: Instantaneous flow tracking algorithm (IFTA) applied to surveillance. Accepted at the Eilat, Israel IEEE Convention 2012
Sung MS, Gjyrezi A, Syed Y, Lannin T, Hristov A, Matov A, Loftus M, Galletti G, Lee GY, Mason C, Kirby B, Nanus DM, Tagawa ST, Giannakakou P. Molecular determinants of taxane activity using circulating tumor cells (CTCs) from castrate-resistant prostate cancer patients. Physical Sciences-Oncology Center on the Microenvironment & Metastasis, New York Academy of Medicine, Understanding Cancer: Genomes to Devices Symposium 2012